Time-lapse imaging of mitotic live bovine zygote expressing EGFP-MAP4 (green) and H2B-mCherry (magenta) after mRNA injection at pronuclear stage. Time resolution, 2.5 min. Scale bar, 10 µm. Movie shows 30 frames/s. Recordings start at 2.5 min before NEBD of the leading PN (PN1). Movies show spindle assembly around the volume of the leading PN (PN1) in one zygote, also depicted in Fig. 3 C and Fig. S4, E and F.
