Time-lapse imaging of mitotic live bovine zygote expressing EGFP-MAP4 (green) and H2B-mCherry (magenta) after mRNA injection at pronuclear stage. Time resolution, 2.5 min. Scale bar, 10 µm. Movie shows 30 frames/s. Recording starts at 2.5 min before NEBD of the leading PN (PN1). Movie shows spindle assembly around the volume of the lagging PN (PN2) in one zygote, also depicted in Figs. 3 C and Fig. S4, E and F.
