Video 2.

Time-lapse imaging of mitotic live bovine zygote expressing EB3-mEGFP2 (green) and H2B-mCherry (magenta) after mRNA injection at pronuclear stage. Time resolution; 2.5 min. Scale bar, 10 µm. Movie shows 30 frames/s. Recording starts at 2.5 min before synchronous NEBD. Movie shows spindle assembly in zygote depicted in Figs. 3 B and S4 D.

Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes

Isabell Schneider, Marta de Ruijter-Villani, M. Julius Hossain, Tom A.E. Stout, and Jan Ellenberg

DOI: 10.1083/jcb.202010106