Time-lapse imaging of mitotic live bovine zygote expressing EGFP-MAP4 (green) and H2B-mCherry (magenta) after mRNA injection at pronuclear stage. Time resolution, 2.5 min. Scale bar, 10 µm. Movie shows 30 frames/s. Recording starts at 2.5 min before NEBD of the leading PN (PN1). Movie shows spindle assembly in zygote depicted in Figs. 3 A and S4 C. It is also an example used for analysis (see Fig. 4, C–F).
