Video 7.

3D animation of all segmented β cells. Animation showing a raw FIB-SEM slice followed by the corresponding segmentation masks and a 3D rendering of the respective reconstructed β cell (high-glucose cell 1) with a transparent plasma membrane, insulin SGs (orange), mitochondria (blue), Golgi apparatus (green), centrioles (purple), nucleus (white), and microtubules (red). The individual organelles are highlighted followed by 3D renderings of all segmented cells. Rendering was done with blender. Videos can be viewed and downloaded via https://betaseg.github.io/.


3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cells

Andreas Müller, Deborah Schmidt, C. Shan Xu, Song Pang, Joyson Verner D’Costa, Susanne Kretschmar, Carla Münster, Thomas Kurth, Florian Jug, Martin Weigert, Harald F. Hess, and Michele Solimena

DOI: 10.1083/jcb.202010039
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