Confocal time-lapse imaging of a COS7 cell coexpressing VPS13D^Halo, mCh-Miro1(ΔTM)-SspB, and EGFP-iLID-Mito (not shown), showing recruitment of VPS13D and Miro1 upon blue light irradiation (at 0 s) of the area indicated by the white box. Images were acquired at a rate of 0.5 frames/s for a total of 4 min. Scale bars, 10 µm.