FRAP of G3BP1-GFP and GFP-ATXN2L within cellular stress granules (SGs). Video corresponds to stills and FRAP curves shown in Fig. 2, F and G. Stable G3BP1-GFP or KI GFP-ATXN2L U2OS cells were exposed to 500 µM sodium arsenite for 30 min. Imaging was performed using time-lapse spinning-disk confocal microscopy of cells adhering to the surface of the imaging chamber. Arrows indicate stress granules on which FRAP was performed. Images were captured every 0.1 s for 35 s total. Photobleaching with 488-nm laser occurred 2 s into capture. Frames per second = 30.
