Formation, growth, and fusion of granules from G3BP1-GFP U2OS lysate induced with purified G3BP1. Video shows a zoomed-out field of the granule shown in Fig. 1 F. Imaging was performed using time-lapse spinning-disk confocal microscopy of granules settled at the surface of the imaging chamber 5 min after induction of granules by increasing the concentration of G3BP1 by 20 µM using purified G3BP1. DIC and G3BP1-GFP images were captured every 0.083 min for 25 min total. Frames per second = 30.
