Video 2.

Dynamics of GBP-TM-mScarlet relocalization by fibrinogen-GFP micropatterns. NIH/3T3 cells stably expressing GBP-TM-mScarlet were allowed to spread on dual micropatterns of fibronectin/fibrinogen-Alexa647 and fibrinogen-biotin-ATTO490LS::streptavidin-GFP-GFP and were then imaged live by TIRF microscopy. Elapsed time in minutes. This movie corresponds to Fig. 6 D. Scale bar, 10 µm. Playback speed: 10 frames per second.


High-efficacy subcellular micropatterning of proteins using fibrinogen anchors

Joseph L. Watson, Samya Aich, Benjamí Oller-Salvia, Andrew A. Drabek, Stephen C. Blacklow, Jason Chin, and Emmanuel Derivery

DOI: 10.1083/jcb.202009063
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