In support of Fig. 2: Centrioles assemble de novo, recruit different centrosomal molecules, and duplicate. Maximum intensity z projection from time-lapse videos of explants from noncycling unfertilized Drosophila eggs overexpressing Plk4, acquired on a spinning-disk confocal microscope. Shown is centriole biogenesis reported by centrosomal protein Ana1 (in green) and the MT-associated protein Jupiter (magenta). Time (minutes:seconds) is shown at the top left. Playback rate is 5 fps.
