Maximum-intensity Z-projection from a 3D time-lapse video of an embryo expressing Klp61F::GFP (green) and Feo::mCherry (magenta). Note that during manipulation there is additional signal from bright-field illumination, which helped visualize the glass cantilever. Time in min:s; scale bar, 30 µm; acquired at 1 frame s−1 in the first part and 2 frames min−1 in the second part; and displayed at 10 frames s−1. In support of Fig. 4.
