3D-reconstructed image of DLD1CENP-A cells without DOX treatment as in Fig. 4 E showing Astrin-positive kinetochores. DLD1CENP-A cells were grown in RPMI media on glass coverslips in a 12-well tissue culture dish for 72 h. Cells were fixing with ice-cold methanol for 1 min. Cells immunostained with antibodies against Astrin and HEC1. 3D rendering was performed with Imaris 9.5.0.
