WT WDR62 N2-GFP recruits p60/TagBFP-p80 to sever curved MTs in cells. MRC5 cells were cotransfected with WT WDR62 N2-GFP, DCX-mCherry, and p60/TagBFP-p80. Arrows indicate that p60/TagBFP-p80 was recruited to curved MT lattices by WDR62 N2 WT-GFP and that breakage occurred. Images were collected with a TIRF microscope at 2-s intervals. Video is sped up 10 times (5 frames/s).
