Video 3.

Example of AI-PS platform for TFEB screen. Nikon NIS elements JOB module screen capture during TFEB-GFP (green) acquisition. Top: TFEB-GFP is in green and red circle is for the phenotype automatic detected cells. Bottom left corner: R-based image segmentation, machine learning prediction, and mask generation. Bottom right: Photoactivation in red (pa-mCh). Three examples are shown here.


Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes

Gil Kanfer, Shireen A. Sarraf, Yaakov Maman, Heather Baldwin, Eunice Dominguez-Martin, Kory R. Johnson, Michael E. Ward, Martin Kampmann, Jennifer Lippincott-Schwartz, and Richard J. Youle

DOI: 10.1083/jcb.202006180
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