Example of AI-PS platform for TFEB screen. Nikon NIS elements JOB module screen capture during TFEB-GFP (green) acquisition. Top: TFEB-GFP is in green and red circle is for the phenotype automatic detected cells. Bottom left corner: R-based image segmentation, machine learning prediction, and mask generation. Bottom right: Photoactivation in red (pa-mCh). Three examples are shown here.