Pericentrin-depleted HeLa cells expressing EGFP-centrin1 and mCherry-NuMA with one centrosome labeled with SiR-tubulin and observed with a 40× objective. Green and gray represent EGFP-centrin1 and SiR-tubulin, respectively. mCherry-NuMA is not shown. 10 × 2.2–μm z-stacks were acquired every 10 min. Playback is sped up 4,200× relative to real time. Image frames shown are maximum intensity projections at each time point. Related to Fig. S1 H.
