Video 3.

DMSO or centrinone-treated RPE1 cells expressing in situ mNeonGreen-tagged CEP192 and transgene-encoded mRFP-H2b. 5 × 2–µm z-stacks were acquired every 5 min at 60× magnification. Image frames shown are maximum intensity projections at each time point. Playback is sped up 900× relative to real-time. Elapsed time shown is in hours:minutes, and scale bar is 10 µm.


Centriole-independent mitotic spindle assembly relies on the PCNT–CDK5RAP2 pericentriolar matrix

Sadanori Watanabe, Franz Meitinger, Andrew K. Shiau, Karen Oegema, and Arshad Desai

DOI: 10.1083/jcb.202006010
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