Representative video of a RUSH assay in a giantin KO RPE1 cell stably expressing GFP-COL1A1 (green) and transiently transfected with mCh-ST (magenta, Golgi label) and an ER RUSH hook. At time 0, biotin and ascorbic acid were added to the medium to release the collagen from the hook and promote procollagen folding, respectively, to trigger anterograde trafficking. Video is cropped to the relevant time frame; time is indicated in top left corner as min:s. Frames were captured as single-frame images every 20 s. Video frame rate: 12 frames/s. Scale bar = 10 µm.
