Chromosome misalignment in Cep57/Cep57L1-depleted cells with precociously disengaged centrioles. HeLa-GFP-centrin1 (green) cells were treated with siCep57 and siCep57L1 in the presence of SiR-DNA (200 nM, red). 25 × 1.2–µm z-stacks were acquired every 10 min at 40× magnification. Image frames shown are maximum-intensity projections at each time point.
