Video 3.

Dynamics of the GFP-tagged version of WT KLP-7 and mutant KLP-7S546E S555E, related to Fig. 9. The time-lapse video shows the movement of GFP::KLP-7 in PLM neurons of WT, unc-73(0), and let502 RNAi. Similarly, a GFP-tagged version of the B-type phosphomutant KLP-7S546E S555E was also imaged. The movements are indicated by green arrows. Videos were captured using a spinning disk confocal microscope at a speed of 2.64 frames per second and played at 15 frames per second.


Wnt signaling establishes the microtubule polarity in neurons through regulation of Kinesin-13

Dharmendra Puri, Keerthana Ponniah, Kasturi Biswas, Atrayee Basu, Swagata Dey, Erik A. Lundquist, and Anindya Ghosh-Roy

DOI: 10.1083/jcb.202005080
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