Tubulation of phagosomes containing mRFP1–E. coli. Macrophages were allowed to internalize mRFP1–E. coli, and imaging was started 180 min after phagocytosis. mRFP1-positive vesicles are pseudocolored to enhance visibility of low-intensity events. Images were acquired at 1 frame every 3 s. Playback rate is 2 frames/s. Still frames from Video 7 are displayed in Fig. 3 B. Scale bar: 2 µm.