Phagosomes containing fluorescent bead clumps undergo fragmentation. Cy5-labeled 0.1-µm beads were clumped using human IgG and were presented to RAW macrophages for 2 h before the cells were moved to a prewarmed (37°C) microscope stage. Images were acquired at a rate of 3 frames/h for a 6-h period. The Cy5 fluorescence intensity is illustrated as a rainbow scale where red is the highest intensity level and blue is the lowest intensity level. Playback rate is 3 frames/s. Still frames from Video 4 are displayed in Fig. S1 D. Scale bar: 2 µm.
