Fission of phagosomes containing ZsGreen–E. coli. Macrophages were allowed to internalize ZsGreen–E. coli, and imaging was started immediately. Left panel shows the macrophages in DIC. Right panel shows ZsGreen fluorescent proteins from E. coli–containing phagosomes and phagosome-derived vesicles. Images were acquired at a rate of 2 frames/h for a 10-h period. Playback rate is 2 frames/s. Video 3 is complementary to Fig. 1 D. Scale bars: 10 µm.
