Video 1.

The PA sensor accumulates at NE herniations (related to Fig. 5 D). Segmentation and isosurface rendering of correlative light and electron tomography of the NLS-PA sensor-mCherry (magenta) in an nup116Δ cell grown at 37°C for 3 h before processing for EM. The ONM is drawn in blue and the INM in teal. Scale bar, 200 nm. Playback speed, 10 frames per second.


Direct binding of ESCRT protein Chm7 to phosphatidic acid–rich membranes at nuclear envelope herniations

David J. Thaller, Danqing Tong, Christopher J. Marklew, Nicholas R. Ader, Philip J. Mannino, Sapan Borah, Megan C. King, Barbara Ciani, and C. Patrick Lusk

DOI: 10.1083/jcb.202004222
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