The PA sensor accumulates at NE herniations (related to Fig. 5 D). Segmentation and isosurface rendering of correlative light and electron tomography of the NLS-PA sensor-mCherry (magenta) in an nup116Δ cell grown at 37°C for 3 h before processing for EM. The ONM is drawn in blue and the INM in teal. Scale bar, 200 nm. Playback speed, 10 frames per second.