Sheet-like protrusions in ARP2-disrupted dHL60 cells. IRSp53-EGFP localization in chemoattractant stimulated (10 nM fMLP) wild type (left), WAVE complex-null (middle), and ARP2-null (right) dHL60 cells. IRSp53 is a marker of both filopodial and lamellipodial protrusions. TIRF-SIM imaging at one frame every 4 s for wild type cells and one frame every 5 s for WAVE complex and ARP2 nulls. Scale bar: 5 µm. This video corresponds to Fig. 7 A.
