TIRF-SIM imaging of IRSp53 and WAVE complex colocalization at a protruding lamellipodium. dHL60 cell expressing IRSp53-EGFP (cyan, left) and Hem1-mCherry (magenta, middle) continually exposed to chemoattractant (10 nM fMLP) imaged with TIRF-SIM at one frame every 5 s. Merged channels (right) show colocalization at lamellipodium edge. Scale bar: 5 µm. This video corresponds to Fig. 6 B, top.
