TIRF-SIM versus sum-projected TIRF-SIM imaging of WAVE complex rings in the absence of actin polymer. dHL60 cell expressing Hem1-EGFP continually exposed to chemoattractant (10 nM fMLP) and acutely treated with latrunculin A (5 µM) at 30 s. To create a conventional resolution (~TIRF) comparison to highlight the requirement of super-resolution microscopy to resolve diffraction-limited WAVE complex puncta as rings, the nine images (three phases × three angles) that construct TIRF-SIM images were sum-projected. TIRF-SIM (left) versus ~TIRF (right) imaging, one frame every 4 s. Scale bar: 5 µm. This video corresponds to Fig. 2, B and C.
