Lattice light sheet and TIRF imaging of WAVE complex at protruding lamellipodia. Left: dHL60 cell expressing Hem1-EGFP (green) continually exposed to chemoattractant (25 nM fMLP) imaged with lattice light sheet at one frame every 4 s. Gray overlay represents the cell boundary defined by cytosolic Hem1. This video corresponds to Fig. 1 C, top right. Right: dHL60 cell expressing Hem1-EGFP (green) and stained with membrane dye (CellMask DeepRed; gray) continually exposed to chemoattractant (10 nM fMLP). Both channels were imaged simultaneously with TIRF at one frame every 2 s. Scale bar: 5 µm. This video corresponds to Fig. 1 C, bottom.
