Example trajectory of a Swi4-mEos3.2 molecule in a live yeast cell nucleus. Video was made using the MTT software (Sergé et al., 2008) on one of the live cell PALM FOV acquisitions. Frametime was 30 ms. Pixel size was 120 nm. Frames are colored from blue to red as time increases. Both free and confined diffusion are apparent in this trajectory.
