The biogenesis of mKate2-FM4-PAUF–containing CARTS at the TGN. HeLa cells expressing mKate2-FM4-PAUF were first incubated at 20°C with D/D solubilizer and cycloheximide for 45 min. Images were acquired continuously after the temperature shift to 37°C with a time interval between frames of 30 s for ∼75 min. N, nucleus.
