Irradiation of a single mitochondrion followed by fusion and repolarization corresponding to Fig. 7 D. OMM is green (left) and TMRE intensity (right) is represented by red hot heatmap. Time-lapse images over 8 min of a live U-2 OS cell transfected with SNAP-OMP-25 and loaded with TMRE. Frames were captured every 3.5 s using a spinning-disk confocal microscope. Playback rate is 7.5 fps.
