New k-fiber plus-ends can be stabilized after k-fiber breakage, related to Fig. 4. Microneedle manipulation of individual k-fiber reveals an example of stabilized bundle plus ends after k-fiber breakage. The microneedle (Alexa 555, yellow) exerts force on the k-fiber (SiR-tubulin, white) for minutes and is removed after the k-fiber breaks (purple arrowheads). The new plus end fragment of the bundle persists for minutes while the fragment attached to the kinetochore is reincorporated into the spindle. This video shows the later time points and response of the cell from Video 3, where t = 0 is the start of microneedle manipulation. Scale bar, 4 µm. Video was collected using a spinning disk confocal microscope at one frame every 10 s. Video has been adjusted to play back at a constant rate of 15 frames/s. Video corresponds to still images from Fig. 4 G.
