GFP-EB3 movement in RPE1 cells at 2 h after IR treatment. Related to Fig. 2 C (2 h after IR). DMSR-related microtubule nucleation capacity under IR treatment was analyzed by live-imaging time-lapse experiments in RPE-1 cells stably expressing GFP-EB3. GFP-EB3 comets associated with the growing microtubule plus ends at 2 h after IR are shown. G1 RPE-1 cells were treated with IR (5 Gy). At 2 h after IR treatment, images were gathered for 30 s at 1-s intervals using Airyscan and time series of Zeiss confocal microscope. The video was made using these images in chronologic order by Adobe Premiere, the video duration was 5 s, and the frame rate was 6 frames per second.
