2P-IVM of the LyzM-GFP mouse footpad at ∼1 h after injection of saline in one footpad and E. coli BPs in the other. Videos are maximum-intensity projections of an image stack acquired for a period of ∼20 min (min:s:ms) in each footpad with a frame rate of 10 frames/s. The vessels were labeled with anti-CD31 (blue). Scale bar = 50 µm.
