Reversal of RNA pol II transcription inhibition results in disappearance of GFP-PRPF38A droplet-like foci adjacent to nuclear speckles. Time-lapse video of a U2OS cell coexpressing GFP-PRPF38A (green) and mCherry-SON (red). Cells were treated for 2 h with DRB to induce GFP-PRPF38A foci and then rinsed with complete growth media before imaging. Arrowheads indicate GFP-PRPF38A foci that disappear as GFP-PRPF38A renters nuclear speckles. Time (top left) in hours:minutes format.
