Video 1.

Data associated with Fig. S3 B. Fld1-GFP localizes to ER subdomains independent of neutral lipid synthesis. Time-lapse images of 4ΔKO yeast cells expressing genomic Fld1-GFP. Stacks of seven images with a step size of 0.5 µm were acquired at an interval of 1 s for 15 s with an exposure of 500 ms, and deconvolved. Single plane images are displayed.


Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis

Vineet Choudhary, Ola El Atab, Giulia Mizzon, William A. Prinz, and Roger Schneiter

DOI: 10.1083/jcb.201910177
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