Superresolution live-cell microscopy of lysosome dynamics in reovirus-infected cells (related to Fig. 7). HeLa cells were transfected with a σ3-GFP plasmid for 24 h and adsorbed with reovirus T1L M1-P208S at an MOI of 10 PFUs/cell for 1 h. At 16 hpi, cells were imaged every 3 s for 30 min using TIRF microscopy to visualize basal reovirus egress zones. Speed, 3 frames per second. The TIRF microscopy movie shows lysosomes stained with LysoTracker (red) and σ3 visualized with the GFP fusion protein (green). Reovirus σ3 concentrates in lysosomes and moves to the cell periphery. In this infected cell, there are two reovirus egress areas in which both lysosome and σ3 signals intensify.
