Video 5.

Hsc70-EGFP fails to be recruited by clathrin-coated vesicles. Bottom surface of a gene-edited AP2-TagRFP^+/+ SUM159 cell transiently expressing Hsc70-EGFP imaged by TIRF microscopy every 1 s for 154 s. The time series shows diffuse cytosolic fluorescence signal and absence of spots corresponding to clathrin-coated vesicles.

Dynamics of Auxilin 1 and GAK in clathrin-mediated traffic

Kangmin He, Eli Song, Srigokul Upadhyayula, Song Dang, Raphael Gaudin, Wesley Skillern, Kevin Bu, Benjamin R. Capraro, Iris Rapoport, Ilja Kusters, Minghe Ma, and Tom Kirchhausen

DOI: 10.1083/jcb.201908142