Video 4.

EGFP-Hsc70 fails to be recruited by clathrin-coated vesicles. Bottom surface of a gene-edited AP2-TagRFP^+/+ SUM159 cell transiently expressing EGFP-Hsc70 imaged by TIRF microscopy every 1 s for 112 s. The time series shows diffuse cytosolic fluorescence signal and absence of dots corresponding to clathrin-coated vesicles.

Dynamics of Auxilin 1 and GAK in clathrin-mediated traffic

Kangmin He, Eli Song, Srigokul Upadhyayula, Song Dang, Raphael Gaudin, Wesley Skillern, Kevin Bu, Benjamin R. Capraro, Iris Rapoport, Ilja Kusters, Minghe Ma, and Tom Kirchhausen

DOI: 10.1083/jcb.201908142