Video 3.

Sequential recruitment of EGFP-Aux1 and TagRFP-GAK to clathrin-coated vesicles. Bottom surface of a SUM159 cell gene-edited for EGFP-Aux1^+/+ and TagRFP-GAK^+/+ was imaged by TIRF microscopy every 1 s for 120 s. To facilitate visualization, the TagRFP channel was shifted laterally by 5 pixels in the right panel.

Dynamics of Auxilin 1 and GAK in clathrin-mediated traffic

Kangmin He, Eli Song, Srigokul Upadhyayula, Song Dang, Raphael Gaudin, Wesley Skillern, Kevin Bu, Benjamin R. Capraro, Iris Rapoport, Ilja Kusters, Minghe Ma, and Tom Kirchhausen

DOI: 10.1083/jcb.201908142