Dynamics of EGFP-GAK recruitment to clathrin-coated vesicles. Bottom surface of a SUM159 cell gene-edited for EGFP-GAK+/+ and CLTA-TagRFP+/+ was imaged by TIRF microscopy every 1 s for 200 s. To facilitate visualization, the EGFP channel was shifted laterally by 5 pixels in the right panel.