Interfering with the function of dynein by overexpression of p50, Kif5C by overexpression of the rigor mutation T94N, or intracellular transport by expression of the chimera Kif1C-5C reduces THN speed. To improve cell tracking, p50 expression experiments were performed in THNs coexpressing H2B. Expressed markers are indicated at the top. Colored dots indicate individual examples of THNs. Elapsed time in hours:minutes. Bars: 10 µm. The video is related to Figs. 4 and 7.