Microtubules mediate the retraction of supernumerary protrusions via Lfc. Lfc+/+ and Lfc−/− DCs were recorded while migrating within a path choice assay toward a soluble CCL19 gradient in 30-s intervals. Note that both genotypes insert multiple protrusions into different channels when reaching the junction point (black arrowheads). Red arrowheads highlight rupturing events and loss of cellular coherence (only observed in Lfc−/− cells). Time in [min:s]. Scale bar, 10 µm. Frame rate, 10 frames per second.
