Polarized Lfc-GFP distribution precedes retraction of explorative protrusions. A DC expressing Lfc-GFP and EB3-mCherry was acquired while migrating under agarose (first part) or within a 3D pillar array (last part) toward a soluble CCL19 gradient in 2-s intervals on an inverted spinning-disk microscope (inverted signal). Purple arrowheads denote persistent diffuse trailing edge Lfc-GFP signal. Orange arrowheads highlight protrusion-retraction accompanied by a change of Lfc-GFP signal distribution. White and black arrowheads indicate filamentous Lfc-GFP signal distribution in protruding areas after repolarization. Time in [min:s]. Scale bar, 10 µm. Frame rate, 20 frames per second.
