In complex environments, MT depolymerization causes loss of coherence. DCs, either untreated (control) or treated with the indicated compounds (nocodazole or double treatment with Y27632 and nocodazole) were recorded in 60-s intervals while migrating within a path choice assay toward a soluble CCL19 gradient. Note that under all conditions, cells insert multiple protrusions into different channels when reaching the junction point (black arrowheads). Red arrowheads highlight rupturing events and loss of cellular coherence (only observed in drug-treated cells). Time in [min:s]. Scale bar, 10 µm. Frame rate, 10 frames per second.