Lfc regulates MT-mediated adhesion resolution. Nocodazole-treated Lfc+/+ and Lfc−/− DCs were acquired while migrating under agarose toward a soluble CCL19 gradient in 20-s intervals on an inverted cell culture microscope. Left panels show nocodazole-treated cells during adhesive migration. Note the loss of directionality in Lfc+/+ DCs and the pronounced elongation of Lfc−/− DCs. Right panels show nocodazole effects during adhesion-independent migration on PEG-coated coverslips. Note the persistent loss of directionality in Lfc+/+ DCs but the restored cell lengths of Lfc−/− DCs. Time in [min:s]. Scale bar, 100 µm. Frame rate, 20 frames per second.
