Repositioning of iLID-mCherry-RAB5 with opto-kinesin KIF1A(1–365)-VVDfast-GFP-SSPB(micro) in a U2OS cell during pulsed global 470-nm illumination. This video corresponds to Fig. 4 H. Total time: 40 min and 35 s. Acquired with 5 s between frames. 300× sped up.