Repositioning of iLID-mCherry-RAB5 with opto-kinesin KIF1A(1–365)-VVDfast-GFP-SSPB(micro) in a U2OS cell during global 470-nm illumination. This video corresponds to Fig. 4 F. Total time: 6 min. Acquired with 1 s between frames. 15× sped up.