Dual-color imaging of KIF1A(1–365)-VVD52C-mVenus-SSPB(micro) activation and repositioning of iLID-mCherry-RAB5 in a U2OS cell during global 470-nm illumination. This video corresponds to Fig. S2. Total time: 10 min and 35 s. Acquired with 5 s between frames. 150× sped up.