Repositioning of iLID-mCherry-RAB5 with opto-kinesin KIF1A(1–365)-VVD52C-GFP-SSPB(micro) in a U2OS cell during global 470-nm illumination. This video corresponds to Fig. 4 A. Total time: 10 min and 35 s. Acquired with 5 s between frames. 150× sped up.