Repositioning of stably expressed iLID-mCherry-RAB11 with opto-kinesin KIF1A(1–365)-VVDfast-mVenus-SSPB(micro) in multiple HeLa Flp-in cells during global 470-nm illumination. This video corresponds to Fig. 6 I. Total time: 27 min. Acquired with 30 s between frames. 300× sped up.